Histone H3 Polyclonal Antibody, Invitrogen™

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.