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Gibco™ DMEM, powder, low glucose, pyruvate DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, Low Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications.

Manufacturer:  Gibco™ 31600091

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Catalog No. 31600091

Requires : Supplementation



  • Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
  • Store at 2°C to 8°C

Modified as follows:

  • With: Low glucose, L-glutamine, sodium pyruvate, phenol red
  • Without: Sodium bicarbonate, HEPES

Using DMEM

  • DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation\

Order Info

Shipping Condition: Room Temperature



Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Room Temperature
50 L
Low Glucose
HeLa, 293, Cos-7, and PC-12
Standard Serum Supplementation
No Sodium Bicarbonate
Phenol Red
Primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
Sodium Pyruvate
Product Certifications


For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.