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Gibco™ DMEM, powder, low glucose, pyruvate DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, Low Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications.

Manufacturer:  Gibco™ LS31600091

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Catalog No. 31600091



Requires : Supplementation

Description

Description

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
No HEPES
50L
Room Temperature
Sodium Pyruvate
L-Glutamine
Low Glucose
Gibco®
Standard Serum Supplementation
No Sodium Bicarbonate
Powder
Phenol Red
SDS
Documents

In vitro diagnostic; Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.