Thermo Scientific™ FastDigest PdiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer.
Manufacturer: Thermo Scientific™ FD1524
Lambda DNA digested with NaeI (PdiI), 0.7% agarose, 4 cleavage sites
FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
- 100% activity of all FastDigest enzymes in the universal buffer
- 100% buffer compatibility with downstream applications
- Complete digestion in 5-15 minutes
- Direct loading on gels
- No star activity
- 176 FastDigest enzymes available
|Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive|
|10x FastDigest Buffer/FastDigest Green Buffer|