Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)
Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).
Manufacturer: Thermo Scientific™ B52
TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.
- Use fresh 1X TBE both for the gel and for the electrophoresis run
- To prepare 1X TBE buffer, add 100mL of 10X TBE buffer to 900mL of deionized water and mix well
- The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
- 89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3
Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for electrophoresis of RNA and DNA fragme.
Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.
|10X TBE Buffer (Tris-borate-EDTA)|
|89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3|
|There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.|