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MilliporeSigma™ Protein Phosphatase, Lambda, Recombinant Protein, E. coli
Supplier: MilliporeSigma™ 53951420KU
Description
Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn2+-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.Specifications
| In 100mM NaCl, 50mM HEPES, 2mM DTT, 0.1mM MnCl2, 0.1mM EGTA, 50% glycerol, 0.01% BRIJ™ 35 Detergent, pH 7.5. | |
| SDS-PAGE | |
| E. coli | |
| Following initial thaw, aliquot and freeze (−70°C). | |
| Recombinant | |
| ≥95% |
| Further dilute in 50mM Tris-HCl, pH 7.8, 5mM DTT, 2mM MnCl2, and 100μg/mL BSA. | |
| 20 kU | |
| Protein Phosphatase, Lambda | |
| RUO | |
| Liquid |