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CD90.1 (Thy-1.1) Mouse anti-Mouse, Rat, PE, Clone: HIS51, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS12090083

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Catalog No. 501128718



Description

Description

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells. Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer′s cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Specifications

Specifications

CD90.1 (Thy-1.1)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01830, P01831
THY1, Thy-1 cell surface antigen, FLJ33325, Thy-1 T-cell antigen, Thy-1 membrane glycoprotein
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Rat
Flow Cytometry
HIS51
PE
Thy1
Liquid
Thy1
IgG2a, kappa
200 μg
4° C, store in dark, DO NOT FREEZE!
Primary
21838, 24832
Documents

For Research Use Only.