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Actin (muscle), Alexa Fluor 488, clone: HHF35, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS53649682

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Catalog No. 501124609



The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


Actin (muscle)
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P68136, P68035, P62738, P68137, P68134, P68033, P62737, P68133, P68032, P62736, Q3ZC07, P68138, P62739
actin, actin alpha 1, actin alpha cardiac, actin alpha cardiac 1, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, alpha, cardiac 1, actin, alpha, vascular smooth muscle, actin, aortic smooth muscle, alpha actin 1, alpha actin 2, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin cardiac, alpha-actin-1, alpha-actin-2, alpha-cardiac actin, alpha-sma, alphac-actin, cell growth-inhibiting gene 46 protein, nemaline myopathy type 3, skeletal alpha actin, smooth muscle alpha-actin
Affinity chromatography
Bovine, Canine, Equine, Guinea Pig, Human, Murine, Non-human Primate, Porcine, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Alexa Fluor 488
IgG1, kappa, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material.
100057678, 100062175, 100065454, 100152267, 100154254, 100716933, 100722917, 100731953, 11459, 11464, 11475, 281592, 29275, 29437, 477587, 478250, 488984, 515610, 533219, 58, 59, 70, 733615, 81633

For Research Use Only.