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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
2

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

3

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

4

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
5

Cytiva IgG Sepharose™ 6 Fast Flow Medium

Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.

6

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

7

Cytiva Sephadex™ G50 Fine Media

Suitable for gel filtration

Height (Metric) 10cm
Content And Storage 4 to 30°C
Bed Volume 9 to 11mg/g
pH Range 2 to 10
Diameter (Metric) Inner 5cm
For Use With (Application) For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids
Max. Pressure min 150 cm/h, bed height 10 cm, column 5 cm i.d.
Particle Size 34 to 208 μm in wet form, 20 to 80 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G50 Media
Sample Type DNA
Support Type Cross-linked Dextran
Height (English) 3.93 in.
9

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
11

Cytiva HiTrap™ Benzamidine FF (high sub) Columns

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Particle Size 90 μm
Ligand Type >12μM p-aminobenzamidine/mL medium
Height (Metric) 2.5cm
Content And Storage 2° to 8°C
pH Range 2 to 8
Product Type Benzamidine FF Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Serine Proteases such as Thrombin
Material Polypropylene
For Use With (Application) Removal or purification of Serine Proteases such as Thrombin
Support Type 4% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
12

Cytiva Sephacryl™ S-400 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

13

Cytiva Sephacryl™ S-300 High Resolution Media

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

14

Cytiva Sephacryl™ S-200 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

15

Cytiva Superdex™ 30 Prep Grade

Offers high resolution with short run times and good recovery