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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

2

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Plage de pH 5 to 10
Hauteur (impériale) 0.98 in.
Hauteur (métrique) 2.5cm
À utiliser avec (équipement) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Type de ligand 10mg Heparin/mL medium
Matériau Polypropylene
Type de produit Heparin HP Column
Granulométrie 34 μm
Pression max. 42psi
Type de support 6% Highly Cross-linked Agarose
Contenu et stockage 4° to 30°C
À utiliser avec (application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
3

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

4

Cytiva HiTrap™ Blue HP Columns

For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins

Plage de pH 4 to 12
Hauteur (impériale) 0.98 in.
Hauteur (métrique) 2.5cm
À utiliser avec (équipement) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Matériau Polypropylene
Type de produit Blue HP Columns
Type d’échantillon Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins
Granulométrie 34 μm
Pression max. 42psi
Type de support 6% Highly Cross-linked Agarose
Contenu et stockage 4° to 30°C
À utiliser avec (application) Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
5

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Plage de pH 3 to 12
Hauteur (impériale) 0.98 in.
Hauteur (métrique) 2.5cm
À utiliser avec (équipement) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Type de ligand 10μM NHS groups/mL medium
Matériau Polypropylene
Type de produit NHS-Activated HP Column
Granulométrie 34 μm
Pression max. 42psi
Type de support 6% Highly Cross-linked Agarose
Contenu et stockage 4° to 8°C
À utiliser avec (application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
6

Cytiva HiTrap™ Benzamidine FF (high sub) Columns

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Plage de pH 2 to 8
Hauteur (impériale) 0.98 in.
Hauteur (métrique) 2.5cm
À utiliser avec (équipement) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Type de ligand >12μM p-aminobenzamidine/mL medium
Matériau Polypropylene
Type de produit Benzamidine FF Column
Type d’échantillon Serine Proteases such as Thrombin
Granulométrie 90 μm
Pression max. 42psi
Type de support 4% Highly Cross-linked Agarose
Contenu et stockage 2° to 8°C
À utiliser avec (application) Removal or purification of Serine Proteases such as Thrombin
7

Cytiva HiTrap™ Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules

8

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Plage de pH 2 to 13
Hauteur (impériale) 11.81 in.
Hauteur (métrique) 30cm
Format Solid Spherical Bead
Volume du lit 4 to 6mL/g in Distilled Water
Type de produit Sephadex G-25 Media
Diamètre (métrique) interne 2.6cm
Type de support Cross-linked Dextran
Contenu et stockage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
À utiliser avec (application) Desalting and Buffer exchange and removal free label in industrial applications
9

Cytiva Sephacryl™ S-400 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

10

Cytiva Sephacryl™ S-300 High Resolution Media

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

11

Cytiva Sephacryl™ S-200 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

12

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

13

Cytiva IgG Sepharose™ 6 Fast Flow Medium

Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.

14

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

15

Cytiva Sephadex™ G50 Fine Media

Suitable for gel filtration

Plage de pH 2 to 10
Hauteur (impériale) 3.93 in.
Débit < Darcy´s Law
Type de produit Sephadex G50 Media
Diamètre (métrique) interne 5cm
Type d’échantillon DNA
Type de support Cross-linked Dextran
Contenu et stockage 4 to 30°C
À utiliser avec (application) For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids
Hauteur (métrique) 10cm
Format Solid Bead
Volume du lit 9 to 11mg/g
Granulométrie 34 to 208 μm in wet form, 20 to 80 μm in dry form
Pression max. min 150 cm/h, bed height 10 cm, column 5 cm i.d.