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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 44 results
1

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
2

Cytiva Superdex 30 Increase Tricon™ 10/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

3
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Cytiva HiLoad™ Superdex™ 200 PG XK Column

Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.

Content And Storage 4 to 30°C, 20% Ethanol
4

Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Height (Metric) 15cm
Content And Storage 4° to 30°C
Bed Volume 19 to 230L
pH Range 3 to 11
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
Max. Pressure 100kPa
Particle Size 25 to 75 μm
Flow Rate >125 cm/hr.
Format Solid Spherical Bead
Product Type Sephacryl HR Media
Sample Type Polysaccharides, macromolecules and small plasmids
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Height (English) 5.9 in.
5

Cytiva HiPrep™ Sephacryl™ S-200 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
6

Cytiva HiPrep™ Sephacryl™ S-100 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Peptides and small proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
7

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

8

Cytiva Sephadex™ G15 Media

Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight

9

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
10

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
11

Cytiva Sephadex™ G10 Media

Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules

Height (Metric) 30cm
Content And Storage 4 to 30°C
Bed Volume 2 to 3mL/g in Distilled Water
pH Range 2 to 13
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W.
Max. Pressure 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
Particle Size 55 to 165 μm in wet form, 40 to 120 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G10 Media
Sample Type Peptides, Small Biomolecules
Support Type Cross-linked Dextran
Height (English) 11.81 in.
12

Cytiva Sephadex™ Ion Exchange Media

Separate molecules over broad molecular weight and pH ranges

Height (Metric) 5 to 15cm
Content And Storage 4° to 30°C
Format Solid Spherical Bead
Product Type Sephadex Ion Exchange Media
For Use With (Equipment) Column or Batch Techniques
For Use With (Application) Separating and purifying Proteins, Polypeptides, Nucleic Acids and other charged biomolecules by using column or batch techniques
Support Type Cross-linked Dextran
Height (English) 2 to 5.9 in.
13

Cytiva HiLoad™ 16/600 Superose™ 6 Prep Grade Preparative SEC Column

A prepacked SEC column for high-resolution purification of large biomolecules and protein complexes with sample volumes up to 5 mL

14

Cytiva Superdex™ 200 Prep Grade

Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D