Gel Filtration Media and Columns
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Cytiva Sephadex G-50 Medium, 500g
Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.
Cytiva Sephacryl™ S-200 High Resolution Media
Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules
| Plage de pH | 3 to 11 |
|---|---|
| Hauteur (impériale) | 23.62 in. |
| Hauteur (métrique) | 60cm |
| À utiliser avec (équipement) | Low to medium pressure AKTA™ systems |
| Matériau | Polypropylene |
| Type de produit | Sephacryl HR Column |
| Type d’échantillon | Antibodies, serum proteins and mid-size proteins |
| Quantité | Each |
| Granulométrie | 25 to 75 μm |
| Pression max. | 1.5 bar [0.15 MPa] (22 psi) |
| Type de support | Allyl Dextran and N, N'-methylenebisacrylamide |
| Contenu et stockage | 4°C to 30°C, 20% Ethanol |
| À utiliser avec (application) | Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix |
Cytiva Superose™ Media Prep Grade Lab Packs
High performance gel filtration media for scaleup to preparative purifications of biomolecules
| Plage de pH | 3 to 12 |
|---|---|
| Débit | 40 cm/hr. |
| Volume du lit | ≈100 mL |
| À utiliser avec (équipement) | AKTA™ design pump or system |
| Type d’échantillon | Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules |
| Quantité | Each |
| Granulométrie | 30 to 40 μm |
| Type de support | Highly Cross-linked Agarose |
| À utiliser avec (application) | Separations of Proteins and other biomolecules according to size |
| Plage de pH | 3 to 11 |
|---|---|
| Hauteur (impériale) | 23.62 in. |
| Hauteur (métrique) | 60cm |
| À utiliser avec (équipement) | Low to medium pressure AKTA™ systems |
| Matériau | Polypropylene |
| Type de produit | Sephacryl HR Column |
| Type d’échantillon | Antibodies, serum proteins and mid-size proteins |
| Quantité | Each |
| Granulométrie | 25 to 75 μm |
| Pression max. | 22psi |
| Contenu et stockage | 4° to 30°C |
| À utiliser avec (application) | Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix |
Cytiva Sephadex™ G-25 Media
Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.
| Plage de pH | 2 to 13 |
|---|---|
| Hauteur (impériale) | 11.81 in. |
| Hauteur (métrique) | 30cm |
| Format | Solid Spherical Bead |
| Volume du lit | 4 to 6mL/g in Distilled Water |
| Type de produit | Sephadex G-25 Media |
| Diamètre (métrique) interne | 2.6cm |
| Type de support | Cross-linked Dextran |
| Contenu et stockage | 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH |
| À utiliser avec (application) | Desalting and Buffer exchange and removal free label in industrial applications |
Cytiva Sephacryl™ S-500 High Resolution Media
Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules
| Plage de pH | 3 to 11 |
|---|---|
| Hauteur (impériale) | 5.9 in. |
| Débit | >125 cm/hr. |
| Type de produit | Sephacryl HR Media |
| Diamètre (métrique) interne | 40 to 140cm |
| Type d’échantillon | Polysaccharides, macromolecules and small plasmids |
| Type de support | Allyl Dextran and N, N'-methylenebisacrylamide |
| Contenu et stockage | 4° to 30°C |
| À utiliser avec (application) | Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale |
| Hauteur (métrique) | 15cm |
| Format | Solid Spherical Bead |
| Volume du lit | 19 to 230L |
| Granulométrie | 25 to 75 μm |
| Pression max. | 100kPa |
Cytiva Superdex™ 75 Prep Grade
Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins
Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids
| Plage de pH | 3 to 11 |
|---|---|
| Hauteur (impériale) | 23.62 in. |
| Hauteur (métrique) | 60cm |
| À utiliser avec (équipement) | Low to medium pressure AKTA™ systems or pumped chromatography systems |
| Matériau | Polypropylene |
| Type de produit | Sephacryl HR Pre-packed Gel Filtration Column |
| Type d’échantillon | Polysaccharides, macromolecules with extended structures and even small plasmids |
| Quantité | Each |
| Granulométrie | 25 to 75 μm |
| Pression max. | 1.5 bar [0.15 MPa] (22 psi) |
| Type de support | Allyl Dextran and N,N'-methylenebisacrylamide |
| À utiliser avec (application) | Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids |
| Plage de pH | 3 to 11 |
|---|---|
| Hauteur (impériale) | 23.62 in. |
| Hauteur (métrique) | 60cm |
| À utiliser avec (équipement) | Low to medium pressure AKTA™ systems |
| Matériau | Polypropylene |
| Type de produit | Sephacryl HR Column |
| Type d’échantillon | Peptides and small proteins |
| Quantité | Each |
| Granulométrie | 25 to 75 μm |
| Pression max. | 1.5 bar [0.15 MPa] (22 psi) |
| Type de support | Allyl Dextran and N, N'-methylenebisacrylamide |
| Contenu et stockage | 4°C to 30°C, 20% Ethanol |
| À utiliser avec (application) | Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix |
| Plage de pH | 2 to 10 |
|---|---|
| Hauteur (impériale) | 3.93 in. |
| Hauteur (métrique) | 10cm |
| Format | Solid Bead |
| Volume du lit | 9 to 11mg/g |
| Type de produit | Sephadex G50 Media |
| Diamètre (métrique) interne | 5cm |
| Type d’échantillon | DNA |
| Granulométrie | 100 to 300 μm in wet form, 50 to 150 μm in dry form |
| Type de support | Cross-linked Dextran |
Cytiva Superdex™ 200 Prep Grade
Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D
Cytiva Sephadex™ G50 Superfine Media
Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.