Protein Modification Reagents

Chemical agents used to modify the amino acid side chains of proteins in order to alter their native charges, block or expose reactive binding sites, inactivate functional groups, and change functional groups to create targets for crosslinking and labeling.

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Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

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Thermo Scientific™ Proteinase K Powder (≥30 U/mg)

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

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MilliporeSigma™ Chymotrypsin, Human Pancreas

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV

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MilliporeSigma™ Proteinase K Solution, 600mAU/mL

Efficient removal of proteins from nucleic acid solutions

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Spécifications

Type de produit	Proteinase K
Contenu et stockage	-20°C

MilliporeSigma™ Calbiochem™ 5'-Deoxy-5'-methylthioadenosine

Inhibits protein carboxymethyltransferase, induces apoptosis in leukemia U937 cells.

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MilliporeSigma™ Calbiochem™ Cleland’s Reagent

Protective agent for SH groups

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Spécifications

Poids moléculaire	154.24
Synonyme	DTT, Dithiothreitol
CAS	12-3-3483
Pureté	≥97% by titration
Type de produit	Calbiochem Cleland’s Reagent
Solubilité	EtOH, Water
Forme	Powder
Contenu et stockage	2°C to 8°C
Formule moléculaire	C4H10O2S2

Thermo Scientific™ MA(PEG)24 Methyl-PEG-Amine Compound

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications.

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MilliporeSigma™ β-Lactamase, Bacillus cereus 569/H9

Useful for assaying penicillins and cephalosporins

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MilliporeSigma™ Calbiochem™ BAPTA/AM

Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA

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Spécifications

Synonyme	1,2-bis(o-Aminophenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Tetra(acetoxymethyl) Ester
CAS	126150-97-8
Pureté	≥90% by TLC
Type de produit	Calbiochem BAPTA/AM
Perméable à la membrane	Y
Solubilité	DMSO
Forme	Solid
Contenu et stockage	-20°C

Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.