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Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Catalog No. PI20290
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Catalog No. Quantity
PI20290 5 g
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Catalog No. PI20290 Supplier Thermo Scientific™ Supplier No. 20290
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Description

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE).

Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE).  5g glass bottle.

Specifications

Description Pierce DTT, Cleland's Reagent
Content And Storage Store at 4°C.
Product Line Pierce
Form Powder
Quantity 5 g
Product Type Dithiothreitol

Frequently Asked Questions (FAQs)

How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Can you please recommend a method for reduction, alkylation, and digestion of an antibody?

Prepare 1 mg/mL antibody in 7.0 M Guanidine HCl, 100 mM Tris, pH 8.3. For reduction, add DTT (Cat. Nos. A39255, 20290) solution at 10 mM final concentration and incubate for 30 mins at room temperature. For alkylation, add sodium iodoacetate at 20 mM final concentration and incubate in the dark for 20 mins at room temperature. Perform buffer exchange followed by digestion using Pierce MS grade Trypsin, Cat. Nos. 90058, 90057 (1:10 enzyme to substrate ratio).

For Research Use Only. Not for use in diagnostic procedures.