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Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Catalog No. PI23030
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25 g
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Catalog No. Quantity
PI23030 25 g
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Catalog No. PI23030 Supplier Thermo Scientific™ Supplier No. 23030
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Description

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Thermo Scientific™ Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

Highlights:

  • Forms covalent bonds with sulfhydryl groups at pH 6.5-7.5
  • Reaction can be monitored by measuring the decrease in absorbance at 300nm

Specifications

Chemical Reactivity Thiol
Content And Storage Upon receipt store at -20°C.
Label or Dye Unreactive Group
Label Type Blocking Groups
Product Line Pierce
Reactive Moiety Maleimide
Spacer Medium (10 to 30 Å)
Form Solid
Quantity 25 g
Solubility Water
Product Type NEM
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For Research Use Only. Not for use in diagnostic procedures.