Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
ElectroMAX Stbl4 Competent Cells are specifically designed for cloning unstable inserts. This strain is a derivative of Stbl2 cells (engineered from JM109/J5 strain).
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.