Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.
MultiShot Mach1™ T1R Chemically Competent E. coli Cells enable rapid, high-efficiency cloning in an automation-ready 96-well format. Fast-growing and phage-resistant, these cells support same-day transformation and streamlined high-throughput workflows.
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.
MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. T7 lysozyme expression suppresses basal T7 expression.
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).