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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Invitrogen™ Tali™ Cell Cycle Kit  DFS Item

Convenient and accurate determination of the percentage of cells in each phase of the cell cycle

2

Invitrogen™ MultiShot™ FlexPlate Mach1™ T1R Competent Cells  DFS Item

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

3

MilliporeSigma™ Annexin V-PE Apoptosis Detection Kit

Identifies phosphatidylserine (PS) that translocates from cytoplasmic face of plasma membrane to cell surface during apoptosis.

4

Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.

Résistance aux antibiotiques des bactéries Yes (Streptomycin)
Clonage d’ADN méthylé Yes (mcrA)
Type de produit Electrocompetent Cells
Plasmide May be used for plasmids >20 kb
Résistant au phage T1 (tonA) No
Espèces E. coli (K12)
Clonage d’ADN instable No
Conditions d’expédition Dry Ice
Compatibilité à haut débit Low
Format Tube
Sélection bleue / blanche Yes (lacZΔM15)
Niveau d’efficacité de la transformation High Efficiency (>1 x 1010 cfu/μg)
Gamme de produits One Shot
Contient l’épisome F No
Réduit la recombinaison Yes (recA13)
Améliore la qualité des plasmides Yes (endA1)
Préparation de l’ADN non méthylé No
5

Invitrogen™ One Shot™ TOP10/P3 Chemically Competent E. coli  DFS Item

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

6

MilliporeSigma™ Novagen™ BL21(DE3) Competent Cells

Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.

Efficacité Guaranteed efficiency: >2 x 107 cfu/μg
Contenu et stockage Store at ≤-70deg;C, Avoid repeated freeze/thaw
7

MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells

BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

Efficacité Guaranteed efficiency: >2 x 107 cfu/μg
Contenu et stockage ≤-70°C
8

MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Efficacité Guaranteed efficiency: >5 x 106 cfu/μg
Contenu et stockage ≤-70°C
9

Invitrogen™ Tali™ Calibration Beads  DFS Item

Align and calibrate Tali™ Image-Based Cytometer every three months

10

Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli  DFS Item

One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

11

MilliporeSigma™ Novagen™ Origami™ 2(DE3) Singles™ Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Efficacité Guaranteed efficiency: 2 x 106 cfu/μg
Contenu et stockage ≤-70°C
12

Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli  DFS Item

The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.

13

Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.

14

Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli  DFS Item

One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).

15

Invitrogen™ MultiShot™ Mach1™ T1R Chemically Competent E. coli Cells, 96-well plate

MultiShot Mach1™ T1R Chemically Competent E. coli Cells enable rapid, high-efficiency cloning in an automation-ready 96-well format. Fast-growing and phage-resistant, these cells support same-day transformation and streamlined high-throughput workflows.