Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.
MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
MultiShot StripWell Stbl3 Chemically Competent E. coli cells are cloning competent cells designed for cloning direct repeats found in lentiviral expression vectors.
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.
MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems.
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).