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Molecular Probes™ Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

Catalog No. 501121562
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Catalog No. 501121562 Supplier Molecular Probes™ Supplier No. V35116
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Description

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes in mitochondrial membrane potential. After staining a cell population with Alexa Fluor™ 488 annexin V and MitoTracker™ Red CMXRos dye in the provided binding buffer, live cells exhibit very little green fluorescence and bright red fluorescence, whereas apoptotic cells exhibit green fluorescence and decreased red fluorescence. These populations can easily be distinguished using a flow cytometer, and the 488 nm line of an argon-ion laser can be used to excite both dyes.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

Quantity 1 kit
Format Tube, Slide
Product Type Mitochondrial Membrane Apoptosis Kit
Content And Storage Contains 1 vial of annexin, Alexa Fluor™ 488 conjugate (250 μL), 3 vials of MitoTracker™ red (50 μg per vial), 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 μL).

Store in refrigerator (2–8°C) and protect from light.
Flow Cytometer Laser Lines 488
Excitation/Emission Mitotracker™ red 579⁄599, Alexa Fluor™ 488: 499⁄521
No. of Reactions 50
Shipping Condition Wet Ice
Product Line Alexa Fluor, MitoTracker
For Use With (Application) Flow Cytometry
Conjugate Alexa Fluor 488, MitoTracker Red
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
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Frequently Asked Questions (FAQs)

What are the fluorescence excitation/emission maxima for the dyes contained in the Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker Red & Alexa Fluor 488 annexin V, for flow cytometry

Alexa Fluor 488 annexin V: 499/521 nm
MitoTracker Red: 579/599 nm
I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

For Research Use Only. Not for use in diagnostic procedures.