missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Apoptosis Reagents and Kits
  6. 501121533

Molecular Probes™ MitoProbe™ Transition Pore Assay Kit, for flow cytometry

Catalog No. 501121533
Encompass
Change view
Click to view available options
Quantity:
1 kit
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
501121533 1 kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 501121533 Supplier Molecular Probes™ Supplier No. M34153
Only null left

Description

MitoProbe™ Transition Pore Assay Kit, for flow cytometry

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

Quantity 1 kit
Format Tube
Product Type Transition Pore Assay Kit
Content And Storage Contains 5 vials of calcein, AM (50 μg of dry powder/vial), 1 vial of cobalt (II) Chloride hexahydrate (1.2 mL, 80 mM in saline), 1 vial of ionomycin (55 μg), calcium salt, and 1 vial of DMSO (1.5 mL).

Store in freezer (-5 to -30°C).
Excitation/Emission 488
No. of Reactions 100 Reactions
Shipping Condition Room Temperature
Product Line MitoProbe
Conjugate Calcein AM

Frequently Asked Questions (FAQs)

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.
What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

For Research Use Only. Not for use in diagnostic procedures.