
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
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Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits
The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | Medium |
No. of Reactions | 100 Reactions |
Product Type | Mycoplasma Detection |
Form | Liquid |
Sample Type | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
Reaction Speed | Standard |
Label or Dye | FAM |
For Use With (Application) | Microbial Detection |
PCR Method | Multiplex qPCR |
Target Organism Class | Mollicutes, mycoplasma |
Product Line | MycoSEQ™ |
Detection Method | Primer-probe |
Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Shipping Condition | Dry Ice |
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Polymerase | Taq DNA Polymerase |
Product Type | One-Step qRT-PCR Kit |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | Platinum™, SuperScript™ |
Detection Method | Primer-probe |
Invitrogen™ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Content And Storage | Store in freezer (-5° to -30°C). |
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Label or Dye | ROX |
Detection Method | Primer-probe |
Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Polymerase | Taq DNA Polymerase |
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Format | Tube |
Product Type | qRT-PCR Kit |
For Use With (Equipment) | 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ |
Sample Type | RNA |
Reaction Speed | Fast or Standard |
PCR Method | 1-step RT-qPCR |
Product Line | SuperScript™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
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TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
GC-Rich PCR Performance | Low |
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Format | Tube(s) |
Internal Probe Modification | TAMRA™ Quencher (3'), VIC™ (5') |
Product Type | Exogenous Internal Positive Control Reagent |
Reaction Speed | Standard |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Applied Biosystems™ Megaplex™ PreAmp Primers, Rodent Pool A
For use when assay sensitivity is of the utmost importance, Applied Biosystems™ Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs.
Content And Storage | Contains 150 μL of 10X primers Store at -15° to -25°C. |
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6-Tamra NHS/DMSO
The single-isomer preparations of TAMRA are most important for high-resolution techniques such as DNA sequencing and capillary electrophoresis
Applied Biosystems™ TaqMan™ Fast Reagent Starter Kit
Designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5' nuclease assay, in fast thermal cycling mode.
Sample Type | DNA (Genomic), cDNA |
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Reaction Speed | Fast |
For Use With (Application) | Gene Expression |
Detection Method | Primer-probe |
Applied Biosystems™ Precision Plate Holder for Plates, for 7500 Fast System
Precision plate holder for 7500 fast system
Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 10 X |
Reverse Transcriptase | MultiScribe™ |
Final Product Type | cDNA |
Concentration | 10X |
For Use With (Application) | miRNA analysis |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Size (Final Product) | 200 bp or less |
Reaction Format | Separate Components |
Product Type | MicroRNA Reverse Transcription Kit |
Sample Type | RNA |
Reaction Speed | Fast |
Optimal Reaction Temperature | 42°C |
Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Bacillus anthracis Detection Kit
TaqMan™ Bacillus anthracis Detection Kit
Applied Biosystems™ TaqMan™ Reagents Starter Kit
TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.
Sample Type | DNA (Genomic), cDNA |
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Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit
TaqMan™ PreAmp Master Mix Kit contains TaqMan™ PreAmp Master Mix and TaqMan™ Gene Expression Master Mix.
Content And Storage | Contains 2X TaqMan PreAmp Master Mix (1 mL), TaqMan™ Gene Expression Master Mix (5 mL), and TaqMan™ PreAmp Master Mix Kit Quick Reference Card. Store master mixes at 2-8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
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For Use With (Equipment) | 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System |
Sample Type | cDNA |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ RNA-to-CT™ 1-Step Kit
Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.
Polymerase | AmpliTaq Gold DNA Polymerase |
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Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
Product Type | One-Step RT-PCR Kit |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | RNA-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Shipping Condition | Wet Ice |
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Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | RNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Run Time | Standard |
Product Line | RNA-to-CT™, SYBR™ |