Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay
The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.
Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer
The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.
Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.
Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits
A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kit, 100 reactions
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
Applied Biosystems™ TaqMan™ Universal DNA Spike-In Control (5X)
TaqMan Universal DNA Spike-In Control serves as an exogenous process control of a known concentration that is spiked into a sample at specific stages throughout the qPCR process.
Invitrogen™ Dry-Ready™ RT-qPCR Kit
Experience the simplicity and efficiency of the air drying process by gently heating materials at moderate temperatures in a simple ventilated oven.
Applied Biosystems™ TaqMan Fast Advanced Master Mix Gene Expression Bundle
TaqMan Fast Advanced Master Mix delivers better sensitivity, greater specificity, and faster results
Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls
VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.
Applied Biosystems™ Path-ID™ Multiplex One-Step RT-PCR Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kits
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines.
Applied Biosystems™ Path-ID™ Multiplex One-Step Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.