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VetMAX™ PEDV/TGEV/SDCoV Kit
Description
The Applied Biosystems VetMAX PEDV/TGEV/SDCoV Kit is a single-well multiplex real-time RT-PCR kit for the identification of the porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and swine delta corona virus (SDCoV) in environmental and feed samples.
• 4 separate tests are run per sample in 1 well—no need to run several PCRs per virus
• The amplification can be run in standard and fast mode, depending on your needs and laboratory workflow
• Instructions included
The kit includes:
• TaqMan Fast Virus 1-Step Master Mix
• VetMAX PEDV/TGEV/SDCoV Primer Probe Mix—for optimized multiplex real-time RT-PCR amplification of PEDV/TGEV/SDCoV RNA targets
• VetMAX PEDV/TGEV/SDCoV/Xeno Control RNA Mix
• VetMAX Xeno Positive Control RNA—serves as an internal positive control for the RNA purification process and a monitor for the presence of PCR inhibitors
Ease of use
All components for running the PCR are included in the kit. The sample preparation workflow has been tested with Applied Biosystems MagMAX sample extraction chemistry and Thermo Fisher KingFisher instruments. The kit has been validated for several sample types, eliminating the need for separate kits for each type. And this real-time PCR kit can be used with different thermocyclers, with the same results and reliability.
Contact us for more information on how to collect environmental samples on the farm or in transport vehicles.
Environmental samples are:
• Feed samples
• Fecal samples collected from floors or walls
• Swiffer pads
• 4 separate tests are run per sample in 1 well—no need to run several PCRs per virus
• The amplification can be run in standard and fast mode, depending on your needs and laboratory workflow
• Instructions included
The kit includes:
• TaqMan Fast Virus 1-Step Master Mix
• VetMAX PEDV/TGEV/SDCoV Primer Probe Mix—for optimized multiplex real-time RT-PCR amplification of PEDV/TGEV/SDCoV RNA targets
• VetMAX PEDV/TGEV/SDCoV/Xeno Control RNA Mix
• VetMAX Xeno Positive Control RNA—serves as an internal positive control for the RNA purification process and a monitor for the presence of PCR inhibitors
Ease of use
All components for running the PCR are included in the kit. The sample preparation workflow has been tested with Applied Biosystems MagMAX sample extraction chemistry and Thermo Fisher KingFisher instruments. The kit has been validated for several sample types, eliminating the need for separate kits for each type. And this real-time PCR kit can be used with different thermocyclers, with the same results and reliability.
Contact us for more information on how to collect environmental samples on the farm or in transport vehicles.
Environmental samples are:
• Feed samples
• Fecal samples collected from floors or walls
• Swiffer pads
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -30°C). |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | Low |
| PCR Method | 1-step RT-qPCR |
| Reaction Speed | Fast or Standard |
| No. of Tests | 100 tests |
| Product Line | VetMAX |
| Quantity | 100 tests |
| Shipping Condition | Dry Ice |
| Sample Type | Swiffer pads and boots, Food & Environmental Samples |
Frequently Asked Questions (FAQs)
What is the master mix used in the VetMAX PED/TGEV/SDCoV Kit?
The master mix used in this kit is the TaqMan Fast Virus 1-Step Master Mix.
What are the probes included in the VetMAX PEDV/TGEV/SDCoV Kit?
The TaqMan assay for PEDV RNA is labeled with LIZ, the TaqMan assay for TGEV is labeled with FAM, the TaqMan assay for SDCoV is labeled with VIC, and the TaqMan assay for the Xeno RNA is labeled with NED. All four TaqMan assays are combined in the VetMAX PEDV/TGEV/SDCoV Primer Probe Mix.
What is the master mix used in the VetMAX PEDV/TGEV/SDCoV Kit?
The master mix used in the VetMAX PEDV/TGEV/SDCoV Kit is the TaqMan Fast Virus 1-Step Master Mix.