Protein Method Blocking Buffers

MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets

Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 20X), with 2% Tween 20

Thermo Scientific™ Pierce™ Clear Milk Blocking Buffer (10X)

Thermo Scientific™ Pierce Clear Milk Blocking Buffer is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

Thermo Scientific™ Blocker™ BSA

Blocker BSA is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.

Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

MilliporeSigma™ HAT Assay Buffer, 5X

For use in the HAT Assay.

Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.

Thermo Scientific™ SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Thermo Scientific Chemicals Tween 20 washing buffer, 1% in PBS (10X)

Thermo Scientific Chemicals Tween 20 washing buffer, 0.5% in PBS (10X)

Invitrogen™ eBioscience™ IHC /ICC Blocking Buffer - Low Protein

Designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. IHC /ICC Blocking Buffer, Low Protein is recommended for tissues and antibodies with average levels of background staining.

Thermo Scientific Chemicals Tween 20 washing buffer, 0.5% in PBS (10X)

Invitrogen™ I-Block™ Protein-Based Blocking Reagent  

Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.

MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide

For Blocking AB143, AB144P Antibodies

Thermo Scientific™ Blocker™ FL Fluorescent Blocking Buffer (10X)

Blocker FL Fluorescent Blocking Buffer is designed to block excess nonspecific binding sites to help reduce background fluorescence in western blotting applications.