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Thermo Scientific™ StartingBlock™ Blocking Buffer

Catalog No. PI37579
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Quantity:
100 mL
1 L
3 x 1 L
Chemical Name or Material:
PBS
PBST
TBS
TBST
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Catalog No. Quantity Chemical Name or Material
PI37579 100 mL TBS
PI37578 100 mL PBS
PI37538 1 L PBS
FER37538X3 3 x 1 L PBS
PI37542 1 L TBS
EN37539 1 L PBST
EN37543 1 L TBST
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Catalog No. PI37579 Supplier Thermo Scientific™ Supplier No. 37579
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Description

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock Blocking Buffer is available in PBS and TBS, with and without Tween 20.

Features of StartingBlock Blocking Buffer include:

  • Strip and re-probe without re-blocking—blots stay blocked even after stripping with Restore Stripping Buffer (Cat. No. 21059)
  • Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum- and biotin-free)
  • Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes; almost instantaneous for polystyrene microplate wells

Specifications

Chemical Name or Material TBS
Concentration 1X
For Use With (Application) Western Blot
Physical Form Liquid
Product Line StartingBlock
Quantity 100 mL
Formulation Proprietary protein formulation in Tris-buffered saline at pH 7.5

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.