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Thermo Scientific™ Blocker™ BLOTTO in TBS

Catalog No. PI37530
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PI37530 1 L
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Catalog No. PI37530 Supplier Thermo Scientific™ Supplier No. 37530
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Description

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific™ Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for using in Western blotting, ELISA, immunohistochemistry and other detection methods.

Highlights:

  • Popular – nonfat milk has been used for many years in a variety of protein methods, although it is not recommended for avidin-based techniques because it contains some endogenous biotin

  • Convenient – supplied as a ready-to-use 1X TBS solution; can be diluted as needed

  • Easy to use – formulated with anti-foaming agent and thimerosal-free preservative

  • Flexible – may be used for multiple applications, including as a diluent for antibody

Specifications

Chemical Name or Material Blocker BLOTTO
Concentration 1X
For Use With (Application) Western Blot
Physical Form Liquid
Product Line Blocker
Quantity 1 L

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

For Research Use Only. Not for use in diagnostic procedures.