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  5. Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific™ Fish Serum Blocking Buffer

Catalog No. PI37527X3
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500 mL
3 x 500 mL
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PI37527X3 3 x 500 mL
PI37527 500 mL
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Catalog No. PI37527X3 Supplier Thermo Scientific™ Supplier No. LSG37527X3
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Description

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples. Fish Serum Blocking Buffer is effective at stabilizing solutions and samples used for antibody-binding interactions, while at the same time minimizing the possibility of cross-reaction with mammalian sample components.

Features of Fish Serum Blocking Buffer include:
• Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
• Convenient—filtered and stabilized in PBS for compatibility with most assay systems
• Easy to use—can be used as supplied or diluted up to 10-fold as needed
• Flexible - may be used for many different applications, including as a diluent for antibodies

Fish serum offers several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of the antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in the experiments. Fish Serum Blocking Buffer is effective at stabilizing solutions and samples used for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. Try it when use of more-standard sera results in background staining in IHC. In addition, this blocker is effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection. It has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes.

Specifications

Chemical Name or Material Fish Serum Blocking Buffer
Concentration 1X
For Use With (Application) ELISA
Physical Form Liquid
Quantity 3 x 500 mL

Frequently Asked Questions (FAQs)

What is the concentration of protein in the Fish Serum Blocking Buffer?

This information is proprietary. The protein concentration in the Fish Serum Blocking Buffer has been optimized for direct usage without further dilution, however if desired, the blocking buffer may be diluted with phosphate-buffered saline. For best results, our recommendation is to empirically determine the optimal concentration to use.