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Thermo Scientific™ Pierce™ Clear Milk Blocking Buffer (10X)

Catalog No. PI37587
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100 mL
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PI37587 100 mL
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Catalog No. PI37587 Supplier Thermo Scientific™ Supplier No. 37587
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Description

Thermo Scientific™ Pierce Clear Milk Blocking Buffer is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

Thermo Scientific™ Pierce Clear Milk Blocking Buffer is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

Additional Information and Online Ordering

The Thermo Scientific Pierce Clear Milk Blocking Buffer (10X) is a proprietary pre-formulated milk solution for blocking excess nonspecific binding sites, reducing background in Western blotting applications and diluting antibodies when used with nitrocellulose and PVDF membranes. Pierce Clear Milk Blocking Buffer (10X) provides lower background, enhanced sensitivity, extended shelf life and reproducible results compared to homemade buffers based on dissolved non-fat dry milk.

Highlights:

  • Excellent stability – stable for 1 year stored at 4°C, unlike typical homemade milk buffers

  • Convenient – concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications

  • Easy to use – no waiting for powdered milk to dissolve with this ready–to–dilute solution

  • Popular – nonfat milk has been used for many years in a variety of protein methods, although it is not recommended for avidin-based techniques because it contains some endogenous biotin

Specifications

Chemical Name or Material Blocking Buffer
Concentration 10X
For Use With (Application) Western Blot
Physical Form Liquid
Quantity 100 mL

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

For Research Use Only. Not for use in diagnostic procedures.