Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Cytiva Amersham™ Hybond™ Membranes
Unsupported 100% pure nitrocellulose membrane validated for use with all ECL Western and protein blotting applications
Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls
Ideal for Western blotting applications where stripping and reprobing are necessary. Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls are hydrophobic PVDF membranes.
Cytiva Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls
Use for robust protein blotting. Cytiva Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Cytiva Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Sheets
Provides exceptional handling characteristics, allowing reprobing
Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane
Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.
Thermo Scientific™ SuperBlock™ Blocking Buffer
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
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MilliporeSigma™ Immobilon™-P PVDF Membrane
Hydrophobic PVDF Transfer Membrane for western blotting
Thermo Scientific™ Blocker™ Casein
Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.
Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, regular size
iBlot Transfer Stacks are used to transfer proteins.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Fisherbrand™ Semidry Blotting Apparatus
Sleek, low-profile system for fast, efficient Western transfers
MilliporeSigma™ Immobilon™-FL PVDF Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.