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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 45 results
1

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

2

MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma

Ready to use blocking reagent for western blotting immunoassays.

3

Invitrogen™ Novex™ Tris-Glycine SDS Buffer Kit  DFS Item

Made with high-purity reagents and are strictly quality controlled

5

Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer  DFS Item

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

6

MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit

Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.

7

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

8

Invitrogen™ XCell II™ Blot Module  DFS Item

Easy transfer of proteins or nucleic acids from mini-gels to membranes

9

Thermo Scientific Chemicals Nitrocellulose stripping buffer (10X)

Form Liquid
Product Type Western Blot Stripping Buffer
Concentration 10X
10

MilliporeSigma™ Calbiochem™ MOWIOL™ 4-88 Reagent

Used for mounting cells on slides for detection of cell-surface antigens.

11

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Content And Storage Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form Liquid
Product Line Restore
12

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Content And Storage Upon receipt store at room temperature.
Form Liquid
Product Line Restore
13

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
14

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007  DFS Item

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer