General Western Blot Reagents

General Western Blot Reagents
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Thermo Scientificâ„¢ Restoreâ„¢ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restoreâ„¢ |
Thermo Scientificâ„¢ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Thermo Scientificâ„¢ SuperSignalâ„¢ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
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Form | Liquid |
Product Type | Western Blot Enhancer |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence, Fluorescence, Colorimetric |
Thermo Scientificâ„¢ Restoreâ„¢ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restoreâ„¢ |
MilliporeSigmaâ„¢ ReBlot Plus Kit and Reagents
Blots can be stripped of antibodies in approximately 15 minutes at room temperature
For Use With (Application) | Western Blot |
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Invitrogenâ„¢ Boltâ„¢ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogenâ„¢ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogenâ„¢ NuPAGEâ„¢ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer
Form | Liquid |
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Product Type | Western Blot Stripping Buffer |
Concentration | 10X |
Invitrogenâ„¢ XCell IIâ„¢ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
MilliporeSigmaâ„¢ Calbiochemâ„¢ MOWIOLâ„¢ 4-88 Reagent
Used for mounting cells on slides for detection of cell-surface antigens.
MilliporeSigmaâ„¢ Calbiochemâ„¢ SignalBoostâ„¢ Immunoreaction Enhancer Kit
Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.
MilliporeSigmaâ„¢ Calbiochemâ„¢ SeaBlockâ„¢ Reagent, Salmon Plasma
Ready to use blocking reagent for western blotting immunoassays.
Invitrogenâ„¢ No-Stainâ„¢ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Shipping Condition | Dry Ice |
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Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
Product Type | Protein Labeling Reagent |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Membrane Compatibility | Nitrocellulose, PVDF |
Green Features | Less hazardous |
Shelf Life | At least 2 years from receipt |
Run Time | 10 minutes |
For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Product Line | Invitrogenâ„¢ |
Invitrogenâ„¢ NuPAGEâ„¢ MOPS SDS Buffer Kit (for Bis-Tris Gels)
All buffers are made with high-purity reagents and are strictly quality controlled