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Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1 – 15 of 33 results
1

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

3

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Product Type ECL Western Blot Substrate
4

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

5

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Sensitivity Low picogram to mid-femtogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Detection Method Chemiluminescence, Fluorescence
6

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal
Detection Method Chemiluminescence
7
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Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
GREENER_CHOICE

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

EDGE
Sensitivity ∼1 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Sensitive Quantitative or Confirmatory Western Blot
8

MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
9

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition Wet Ice
Substrate Horseradish Peroxidase
Sensitivity Low-femtogram to high-attogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence
10

Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

11

MilliporeSigma™ Immobilon™ Block Noise Canceling Reagents

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Sufficient For Nineteen 8 x 7cm miniblots
Quantity 500 mL
12

Thermo Scientific™ BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine)

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate solutions for chromogenic detection of alkaline phosphatase probes.

13

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

15

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.