
Protein Method Blocking Buffers
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Thermo Scientificâ„¢ SuperBlockâ„¢ Blocking Buffer
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
Recommended Storage | Upon receipt store at 4°C. Product is shipped at ambient temperature. |
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For Use With (Application) | ELISA |
Thermo Scientificâ„¢ StartingBlockâ„¢ Blocking Buffer
StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.
Physical Form | Liquid |
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Recommended Storage | Upon receipt store at 4°C. |
Thermo Scientificâ„¢ Blockerâ„¢ FL Fluorescent Blocking Buffer (10X)
Blocker FL Fluorescent Blocking Buffer is designed to block excess nonspecific binding sites to help reduce background fluorescence in western blotting applications.
Thermo Scientificâ„¢ Blockerâ„¢ BSA
Blocker BSA is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.
Physical Form | Liquid |
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Recommended Storage | Store at 4°C. |
Concentration | 10% BSA |
For Use With (Application) | Western Blot |
Thermo Scientificâ„¢ Fish Serum Blocking Buffer
Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.
Physical Form | Liquid |
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Chemical Name or Material | Fish Serum Blocking Buffer |
Recommended Storage | Upon receipt store at 4°C. |
Concentration | 1X |
For Use With (Application) | ELISA |
Thermo Scientificâ„¢ Blockerâ„¢ Casein
Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.
Physical Form | Liquid |
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Recommended Storage | Store at 4°C. |
Concentration | 1X |
For Use With (Application) | Western Blot |
Invitrogenâ„¢ eBioscienceâ„¢ IHC /ICC Blocking Buffer - Low Protein
Designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. IHC /ICC Blocking Buffer, Low Protein is recommended for tissues and antibodies with average levels of background staining.
Invitrogenâ„¢ eBioscienceâ„¢ IHC/ICC Blocking Buffer - High Protein
Designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. IHC/ICC Blocking Buffer, High Protein is recommended for tissues and antibodies with high background staining, nuclear antigens, and FFPE tissue.
MilliporeSigmaâ„¢ Calbiochemâ„¢ PBS-TWEENâ„¢ Tablets
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C
TBS with Tweenâ„¢ (TBST), 20X Solution, Molecular Biology Grade, Ultrapure
Used in Western blotting protocols
Physical Form | Solution |
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Chemical Name or Material | TBS with Tween (TBST) |
Recommended Storage | Ambient temperatures |
Concentration | 20X |
MilliporeSigmaâ„¢ Chemiconâ„¢ Choline Acetyltransferase Blocking Peptide
For Blocking AB143, AB144P Antibodies
Physical Form | Viscous Liquid |
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Chemical Name or Material | Buffer |
Recommended Storage | Keep cold |
Concentration | 1% in PBS (10X) |
Physical Form | Liquid |
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Chemical Name or Material | Buffer |
Recommended Storage | Keep Refrigerated |
Concentration | 0.5% in PBS (10X) |
Physical Form | Liquid |
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Chemical Name or Material | Blocking Buffer |
Concentration | 0.5% in PBS (10X) |