Protein Method Blocking Buffers

MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide

For Blocking AB143, AB144P Antibodies

MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets

Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C

MilliporeSigma™ Calbiochem™ BLOT-QuickBlocker™ Reagent

Prevents nonspecific proteins from binding to blots.

MilliporeSigma™ HAT Assay Buffer, 5X

For use in the HAT Assay.

Thermo Scientific™ Pierce™ Fast Wash Buffer, 10X

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.

Invitrogen™ eBioscience™ IHC /ICC Blocking Buffer - Low Protein

Designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. IHC /ICC Blocking Buffer, Low Protein is recommended for tissues and antibodies with average levels of background staining.

Thermo Scientific™ Blocker™ BSA

Blocker BSA is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.

Thermo Scientific™ Blocker™ FL Fluorescent Blocking Buffer (10X)

Blocker FL Fluorescent Blocking Buffer is designed to block excess nonspecific binding sites to help reduce background fluorescence in western blotting applications.

Thermo Scientific™ SuperBlock™ Blocking Buffer, blotting

Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis.

Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.

Thermo Scientific™ Pierce™ Fast Blocking Buffer

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.

Thermo Scientific™ Blocker™ Casein

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.

TBS with Tween™ (TBST), 20X Solution, Molecular Biology Grade, Ultrapure

Used in Western blotting protocols

Thermo Scientific Chemicals Tween 20 washing buffer, 0.5% in PBS (10X)

Invitrogen™ I-Block™ Protein-Based Blocking Reagent  

Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.