Cannabis Microbial Testing
Fisherbrand™ Standard Disposable Transfer Pipettes, Nongraduated; Length: 5.875 in.; Capacity: 7.7mL
Single squeeze draws up to 3.2mL
Fisherbrand™ Premium Microcentrifuge Tubes: 1.5mL
Available in eight colors or in assorted-color packs.
Fisherbrand™ Sterile Polystyrene Disposable Serological Pipets with Magnifier Stripe
Pipets have improved optical clarity — high-contrast black markings make volumes easier to read
Denatured Alcohol, 95%, Fisherbrand™, HistoPrep™
CAS: 64-17-5 Molecular Formula: C2H6O Molecular Weight (g/mol): 46.07 InChI Key: LFQSCWFLJHTTHZ-UHFFFAOYSA-N Synonym: 2-hydroxypropane, 2-propanol, alkolave, avantine, dimethylcarbinol, hartosol, isopropanol, isopropyl alcohol, petrohol, sec-propyl alcohol PubChem CID: 702 ChEBI: CHEBI:16236 IUPAC Name: propan-2-ol SMILES: CC(C)O
Fisherbrand™ Disposable Borosilicate Glass Pasteur Pipets
Constriction near top prevents plugs from slipping into pipette body
Fisherbrand™ Disposable Borosilicate Glass Tubes with Plain End
Use these tubes for tissue culture and clinical chemistry applications. Fisherbrand™ Disposable Borosilicate Glass Tubes with Plain End are made of borosilicate glass to reduce pH changes and contaminants potentially leached from soda lime glass.
Fisherbrand™ Safety Bulb Pipette Filler, Red
Fill any standard size pipet without the risk of cracking or chipping. Fisherbrand™ Safety Bulb Pipet Filler uses an ABS attachment with a silicone adapter that seals around pipets without twisting. Comes with an elastic band for convenient hanging on bottles or wrist.
PYREX™ Griffin Beakers
Offers optimum balance between thermal shock resistance and mechanical strength
Falcon™ Disposable Polystyrene Serological Pipets, Sterile
Features exceptionally crisp, dark, easy-to-read printing with reverse and negative graduations. Corning™ Falcon™ Disposable Polystyrene Serological Pipets are individually wrapped.
Fisherbrand™ Sharps-A-Gator™ Sharps Containers
Puncture-resistant with wide rectangular opening for horizontal loading of needles
Corning™ Stripette™ Paper-and-Plastic Wrapped, Polystyrene Serological Pipettes
Manufactured with optically clear polystyrene for accurate measurement. Corning™ Stripette™ Individually Wrapped Plastic or Paper/Plastic Disposable Polystyrene Serological Pipets are ideal for sterile tissue culture applications, especially while wearing latex gloves, as the wrap reduces static cling.
Invitrogen™ SYBR™ Safe DNA Gel Stain
Highly sensitive stain for visualization of DNA in agarose or acrylamide gels
Fisherbrand™ Pipet Controller
A powerful, lightweight and ergonomically designed pipetting controller for all commonly used glass or plastic pipettes
Fisher BioReagents™ Agar, Powder / Flakes, Fisher BioReagents™
Used as a solidifying agent for culture media
Thermo Scientific™ Oxoid™ AnaeroGen™ 2.5L Sachet
Thermo Scientific™ Oxoid AnaeroGen 2.5L Sachets are anaerobic gas generating sachets for use with Thermo Scientific™ Oxoid 2.5L jar, Part No. AG0025A (not included).
Fisherbrand™ SureOne™ Micropoint Pipette Tips, Universal Fit, Non-Filtered
New, improved Fisherbrand SureOne Reload system offers a full line of micropoint universal fit pipet tips
BD Bacto™ Tryptic Soy Broth (Soybean-Casein Digest Medium)
Conforms with USP and EP specifications. BD™ Bacto™ Tryptic Soy Broth (Soybean-Casein Digest Medium) is a dehydrated, general purpose media for isolation, detection and cultivation of wide variety of microorganisms.
Axygen™ Aerosol Filter Tips, Sterile
Prevent cross-contamination with robotic barrier tips. Axygen™ Aerosol Filter Tips, Sterile are e-beam irradiated to prevent product degradation, comply with USP 3127 (Heavy Metal Contamination) and are free of leachable additives. Available in a wide variety of tip styles.
Axygen™ PCR Tubes with Flat Cap, 0.2mL
Designed to fit standard 96-well blocks. Axygen™ PCR Tubes with Flat Cap, 0.2m are constructed of non-sterile polypropylene. Each conical tube features thin and consistent wall thickness. Tight-fitting cap secures contents and prevents evaporation. Available in an assortment of colors.
Cannabis microbial testing is performed to make sure that cannabis plant materials and products are safe for human consumption. This is especially important for medicinal marijuana use because patients may already be ill or immunocompromised. But even recreational users could be harmed by the presence of human pathogens.
Gamma irradiation of plants and products will destroy most bacteria but may not affect viruses or mycotoxins.
Conventional microbiological testing methods can be borrowed from the food and tobacco industries, where techniques are established and widely applied.
- TAMC and TYMC: Total aerobic microbial count (TAMC) and total yeast/mold count (TYMC) estimates the number of viable aerobic bacteria, yeast/mold organisms, or spores in a sample
- Microbiology media, including selective growth media: Broth and agar products formulated to help identify specific species of microorganisms
These methods are not especially sensitive or specific, but they are familiar. Some total count tests offer preliminary results but do not help determine whether a product is safe.
Some molecular-based detection methods are becoming available and are gaining acceptance. Quantitative real-time PCR (qPCR) protocols can identify and quantify microbes in cannabis without the need to culture.
Microbial testing may also help to determine the plant’s microbiome and understand its effects on growth, pathogen resistance, or metabolic activity. This knowledge could lead to greater crop yields.
Cannabis microbial testing procedures may also detect:
- Salmonella ssp.
- Total Coliforms
- Escherichia coli and Shiga toxin-producing E. coli
- Staphylococcus aureus
- Various species of yeast and mold
- Aspergillus ssp. (A. flavus, A. fumigatus, A. niger, A. terreus)