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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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Keyword Search:
1 – 15 of 44 results
1

Cytiva Superdex™ 200 Prep Grade

Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

2

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
3

Cytiva HiTrap™ Benzamidine FF (high sub) Columns

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Particle Size 90 μm
Ligand Type >12μM p-aminobenzamidine/mL medium
Height (Metric) 2.5cm
Content And Storage 2° to 8°C
pH Range 2 to 8
Product Type Benzamidine FF Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Serine Proteases such as Thrombin
Material Polypropylene
For Use With (Application) Removal or purification of Serine Proteases such as Thrombin
Support Type 4% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
4

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

5

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
6

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

7

Cytiva HiTrap™ Blue HP Columns

For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins

Particle Size 34 μm
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 4 to 12
Product Type Blue HP Columns
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins
Material Polypropylene
For Use With (Application) Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
8

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Height (Metric) 30cm
Content And Storage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Bed Volume 4 to 6mL/g in Distilled Water
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex G-25 Media
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange and removal free label in industrial applications
Support Type Cross-linked Dextran
Height (English) 11.81 in.
9

Cytiva Superdex 30 Increase Precision 3.2/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

10

Cytiva HiPrep™ Sephacryl™ S-300 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4° to 30°C
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Max. Pressure 22psi
Height (English) 23.62 in.
11

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

12

Cytiva HiPrep™ Sephacryl™ S-200 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
13

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100 mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose
14

Cytiva HiLoad™ Superdex™ 200 PG XK Column

Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.

Content And Storage 4 to 30°C, 20% Ethanol
15

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl