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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 44 results
1

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

2

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
3

Cytiva IgG Sepharose™ 6 Fast Flow Medium

Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.

4

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

5

Cytiva Sephadex™ G50 Fine Media

Suitable for gel filtration

Height (Metric) 10cm
Content And Storage 4 to 30°C
Bed Volume 9 to 11mg/g
pH Range 2 to 10
Diameter (Metric) Inner 5cm
For Use With (Application) For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids
Max. Pressure min 150 cm/h, bed height 10 cm, column 5 cm i.d.
Particle Size 34 to 208 μm in wet form, 20 to 80 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G50 Media
Sample Type DNA
Support Type Cross-linked Dextran
Height (English) 3.93 in.
6

Cytiva HiTrap™ Benzamidine FF (high sub) Columns

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Particle Size 90 μm
Ligand Type >12μM p-aminobenzamidine/mL medium
Height (Metric) 2.5cm
Content And Storage 2° to 8°C
pH Range 2 to 8
Product Type Benzamidine FF Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Serine Proteases such as Thrombin
Material Polypropylene
For Use With (Application) Removal or purification of Serine Proteases such as Thrombin
Support Type 4% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
7

Cytiva Superdex™ 30 Prep Grade

Offers high resolution with short run times and good recovery

8

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

9

Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

10

Cytiva Sephadex™ G-100 Media

Commonly used for desalting and buffer exchange of very large biomolecules

Content And Storage 4° to 30°C
Bed Volume 15 to 20mL/g in Distilled Water
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G-100 Media
Sample Type Large biomolecules
Quantity 100 g
For Use With (Application) Useful for desalting and Buffer exchange of very large biomolecules
Support Type Cross-linked Dextran
Max. Pressure 0.096 bar
11

Cytiva Superdex™ 200 Increase 3.2/300 Prepacked Precision Column

Used for micro-scale (μg) preparative purification and for characterization and analysis of small volumes (4 to 50μL) of proteins. Cytiva Lifescience™ Superdex™ 200 Increase 3.2/300 Prepacked Precision Column desigened for size exclusion chromatography.

12

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Particle Size 25 to 75 μm
Height (Metric) 60cm
pH Range 3 to 11
Product Type Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Quantity Each
Material Polypropylene
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
13

Cytiva HiLoad™ Superdex™ 200 PG XK Column

Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.

Content And Storage 4 to 30°C, 20% Ethanol
14

Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Height (Metric) 15cm
Content And Storage 4° to 30°C
Bed Volume 19 to 230L
pH Range 3 to 11
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
Max. Pressure 100kPa
Particle Size 25 to 75 μm
Flow Rate >125 cm/hr.
Format Solid Spherical Bead
Product Type Sephacryl HR Media
Sample Type Polysaccharides, macromolecules and small plasmids
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Height (English) 5.9 in.
15

Cytiva Superdex™ 75 Prep Grade

Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins