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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 44 results
1

Cytiva HiPrep™ Sephacryl™ S-200 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
2

Cytiva HiPrep™ Sephacryl™ S-100 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Peptides and small proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
3

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100 mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose
4

Cytiva HiPrep™ Sephacryl™ S-300 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4° to 30°C
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Max. Pressure 22psi
Height (English) 23.62 in.
5

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

6

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Height (Metric) 30cm
Content And Storage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Bed Volume 4 to 6mL/g in Distilled Water
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex G-25 Media
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange and removal free label in industrial applications
Support Type Cross-linked Dextran
Height (English) 11.81 in.
7

Cytiva Sephacryl™ S-300 High Resolution Media

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

8

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

9

Cytiva Superdex™ 200 Increase 3.2/300 Prepacked Precision Column

Used for micro-scale (μg) preparative purification and for characterization and analysis of small volumes (4 to 50μL) of proteins. Cytiva Lifescience™ Superdex™ 200 Increase 3.2/300 Prepacked Precision Column desigened for size exclusion chromatography.

10

Cytiva HiLoad™ Superdex™ 200 PG XK Column

Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.

Content And Storage 4 to 30°C, 20% Ethanol
11

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
12

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Particle Size 25 to 75 μm
Height (Metric) 60cm
pH Range 3 to 11
Product Type Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Quantity Each
Material Polypropylene
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
13

Cytiva Superdex™ 30 Prep Grade

Offers high resolution with short run times and good recovery

14

Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Height (Metric) 15cm
Content And Storage 4° to 30°C
Bed Volume 19 to 230L
pH Range 3 to 11
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
Max. Pressure 100kPa
Particle Size 25 to 75 μm
Flow Rate >125 cm/hr.
Format Solid Spherical Bead
Product Type Sephacryl HR Media
Sample Type Polysaccharides, macromolecules and small plasmids
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Height (English) 5.9 in.
15

Cytiva Superdex™ 75 Prep Grade

Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins