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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
2

Cytiva HiTrap™ Blue HP Columns

For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins

Particle Size 34 μm
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 4 to 12
Product Type Blue HP Columns
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins
Material Polypropylene
For Use With (Application) Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
3

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

4

Cytiva Sephacryl™ S-200 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

5

Cytiva Sephadex™ G-100 Media

Commonly used for desalting and buffer exchange of very large biomolecules

Content And Storage 4° to 30°C
Bed Volume 15 to 20mL/g in Distilled Water
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G-100 Media
Sample Type Large biomolecules
Quantity 100 g
For Use With (Application) Useful for desalting and Buffer exchange of very large biomolecules
Support Type Cross-linked Dextran
Max. Pressure 0.096 bar
7

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
9

Cytiva Sephacryl™ S-300 High Resolution Media

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

10

Cytiva Superdex™ 30 Prep Grade

Offers high resolution with short run times and good recovery

11

Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

12

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Height (Metric) 30cm
Content And Storage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Bed Volume 4 to 6mL/g in Distilled Water
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex G-25 Media
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange and removal free label in industrial applications
Support Type Cross-linked Dextran
Height (English) 11.81 in.
13

Cytiva Superdex™ 200 Increase 3.2/300 Prepacked Precision Column

Used for micro-scale (μg) preparative purification and for characterization and analysis of small volumes (4 to 50μL) of proteins. Cytiva Lifescience™ Superdex™ 200 Increase 3.2/300 Prepacked Precision Column desigened for size exclusion chromatography.

14

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Particle Size 25 to 75 μm
Height (Metric) 60cm
pH Range 3 to 11
Product Type Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Quantity Each
Material Polypropylene
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
15

Cytiva Superdex 30 Increase Tricon™ 10/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL