
General Cell Based Assays
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Thermo Scientificâ„¢ LanthaScreenâ„¢ TR-FRET ER alpha Coactivator Assay Kit
The LanthaScreenâ„¢ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment.
LanthaScreenâ„¢ TR-FRET Progesterone Receptor Coactivator Assay Kit
LanthaScreenâ„¢ TR-FRET Progesterone Receptor Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential progesterone receptor (PR) ligands as agonists or antagonists of ligand-dependent coactivator recruitment.
MilliporeSigmaâ„¢ Chemiconâ„¢ Mast Cell Degranulation Assay Kit
Activity assay, Evaluation of tryptase activity in culture supernatants, cell lysates or other tryptase-containing samples
Invitrogenâ„¢ LiveBLAzerâ„¢ FRET-B/G Loading Kit with CCF2-AM
GeneBLAzerâ„¢ cell-based assays utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4.
Substrate | CCF2-AM |
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Cell Permeability | Cell-Permeant |
Type | FRET-B/G Loading Kit |
Substrate Properties | Chemical Substrate |
For Use With (Equipment) | Flow Cytometer, Microplate Reader |
Substrate Type | Beta-Lactamase Substrate |
Product Line | LiveBLAzerâ„¢ |
Detection Method | Fluorescence |
Invitrogenâ„¢ LiveBLAzerâ„¢ FRET-B/G Loading Kit with CCF4-AM
LiveBLAzerâ„¢ FRET-B/G Loading Kit with CCF4-AM
Substrate | CCF4-AM |
---|---|
Cell Permeability | Cell-Permeant |
Type | FRET-B/G Loading Kit |
Substrate Properties | Chemical Substrate |
Content And Storage | GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B. |
Substrate Type | Beta-Lactamase Substrate |
Product Line | LiveBLAzerâ„¢ |
Detection Method | Fluorescence |
MilliporeSigmaâ„¢ Chemiconâ„¢ Cellular senescence assay
Efficiently detects SA-beta-gal activity at pH 6.0
MilliporeSigmaâ„¢ Chemiconâ„¢ Apo-Direct TUNEL Assay Kit
For labeling DNA breaks to detect apoptotic cells by flow cytometry
MilliporeSigmaâ„¢ Chemiconâ„¢ DNA Ladder Detection Kit
Provides a fast & easy means for detection of DNA fragmentation in apoptotic cells
MilliporeSigmaâ„¢ Chemiconâ„¢ MitoLightâ„¢ Mitochondrial Apoptosis Detection Kit
For flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker
Includes | 10X Incubation Buffer, MitoLight |
---|---|
Content And Storage | -20°C |
Description | Mitochondrial Apoptosis Detection Kit |
For Use With (Application) | For flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker |
Detection Method | Fluorescence |
MilliporeSigmaâ„¢ Chemiconâ„¢ CaspaTag Caspase 3,7 In Situ Assay Kit, Fluorescein
For detection of active caspase-3 or caspase-7 in cells undergoing apoptosis
Includes | Hoechst Stain, FLICA Reagent, 10X Wash Buffer, Fixative, Propidium Iodide |
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Content And Storage | 2°C to 8°C |
Description | CaspaTag Caspase 3,7 In Situ Assay Kit |
For Use With (Application) | For detection of active caspase-3 or caspase-7 in cells undergoing apoptosis |
Detection Method | Fluorescence |
MilliporeSigmaâ„¢ Chemiconâ„¢ Caspase 3 Colorimetric Activity Assay Kit, DEVD
Provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD
MilliporeSigmaâ„¢ Chemiconâ„¢ Caspase 8 Colorimetric Assay Kit, IETD
Provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD
MilliporeSigmaâ„¢ Chemiconâ„¢ CaspaTag Caspase 9 In Situ Assay Kit, Fluorescein
For detection of active caspase-9 in cells undergoing apoptosis
Includes | Hoechst Stain, FLICA Reagent, 10X Wash Buffer, Fixative, Propidium Iodide |
---|---|
Content And Storage | 2°C to 8°C |
Description | CaspaTag Caspase 9 In Situ Assay Kit |
For Use With (Application) | For detection of active caspase-9 in cells undergoing apoptosis |
Detection Method | Fluorescence |
MilliporeSigmaâ„¢ Chemiconâ„¢ Caspase 9 Colorimetric Activity Assay Kit, LEHD
Provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD