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MilliporeSigma™ Fluorescent Mouse ES/iPS Cell Characterization Kit
Contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem and induced pluripotent Stem cells
Supplier: MilliporeSigma™ SCR077
Includes:
- Fast Red Violet solution: One 15mL bottle
- Napthol AS-BI phosphate solution. One 15mL bottle
- Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
- Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
- Mouse anti-DPPA-2, clone 6C1.2, Alexa Fluor™ 488 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
- Mouse anti-SSEA-1, clone MC-480, Cy3 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
- DAPI, 100μL. One vial containing 100μL volume
Description
- Contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse ES/iPS cells
- Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and DPPA-2 and cell surface epitope SSEA-1 to enable rapid immunocytochemical marker analysis
- The Dapi nuclear dye is conveniently included to aid in cell quantification
- While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics
Specifications
| Store at 2° to 8°C until expiration date of kit | |
| Immunocytochemistry | |
| 1 Kit |