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MilliporeSigma™ Fluorescent Mouse ES/iPS Cell Characterization Kit

Contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem and induced pluripotent Stem cells

Supplier:  MilliporeSigma™ SCR077

Catalog No. SCR077



Includes:
  • Fast Red Violet solution: One 15mL bottle
  • Napthol AS-BI phosphate solution. One 15mL bottle
  • Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
  • Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
  • Mouse anti-DPPA-2, clone 6C1.2, Alexa Fluor™ 488 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
  • Mouse anti-SSEA-1, clone MC-480, Cy3 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
  • DAPI, 100μL. One vial containing 100μL volume

Description

Description

  • Contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse ES/iPS cells
  • Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and DPPA-2 and cell surface epitope SSEA-1 to enable rapid immunocytochemical marker analysis
  • The Dapi nuclear dye is conveniently included to aid in cell quantification
  • While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics
Specifications

Specifications

Store at 2° to 8°C until expiration date of kit
Immunocytochemistry
1 Kit
SDS
Documents

Documents

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