Protein Modification Reagents
Invitrogen™ DTT, 1M
DTT, 1M
Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane)
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
MilliporeSigma™ Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus
Serine protease that specifically hydrolyzes peptide bonds at carboxylic side of glutamic acid and aspartic acid residues
Thermo Scientific™ Staphylococcus aureus V-8 Protease
Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.
MilliporeSigma™ Calbiochem™ Cleland’s Reagent
Protective agent for SH groups
MilliporeSigma™ Calbiochem™ DM-NITROPHEN™ Reagent, Tetrasodium Salt
Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis
Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent
A three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent PEGylation of primary amines on proteins or assay surfaces.
Thermo Scientific™ Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)
Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
MilliporeSigma™ Dipeptidylpeptidase IV, His·Tag™, Human, Recombinant, S. frugiperda
Inhibition of DPPIV has been reported to be effective treatment for type II diabetes
MilliporeSigma™ Calbiochem™ EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated
Suitable for use in molecular biology applications