Direct PCR Reagents and Kits
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Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.
Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| Vitesse de réaction | 7 min. |
| Type de produit | Fast Advanced Cells-to-Ct Kit |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | Lysate |
| Méthode PCR | 2-step RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
| Compatibilité à haut débit | High-throughput Compatible |
| Type de réactif | Cell Lysis |
| Fidélité (par rapport à Taq) | 2X |
| Format | Tube |
| Gamme de produits | Cells-to-CT |
| Matériau de départ | 10-100,000 Cells |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | TaqMan |
| Vitesse de réaction | 7 min. |
| Type de produit | Fast Advanced Cells-to-Ct Kit |
| Type d’échantillon | Mammalian cells |
| Cible de purification | Lysate |
| Méthode PCR | 2-step RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
| Compatibilité à haut débit | High-throughput Compatible |
| Type de réactif | Cell Lysis |
| Fidélité (par rapport à Taq) | 2X |
| Format | Tube |
| Gamme de produits | Cells-to-CT |
| Matériau de départ | 10-100,000 Cells |
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
|---|---|
| Durée de fonctionnement | 5 min. |
| Format | Spin Column |
| Contenu et stockage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
| Température de réaction optimale | 42°C |
|---|---|
| Gamme de produits | Ambion, Cells-to-cDNA |
| Type d’échantillon | Mammalian Cells |
| Type de produit final | cDNA |
| Taille (produit final) | 7 kb or less |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | M-MLV |
| Temps de purification | 2 hr. |
| À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
| Contenu et stockage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
| Type de produit | Multiplex Kit |
| Gamme de produits | SNaPshot |
| Type d’échantillon | DNA |
| Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
Invitrogen™ Cells-to-CT Bulk RT Reagents
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Fast or Standard |
| Gamme de produits | CellsDirect, Platinum, SuperScript |
| Type d’échantillon | Cells |
| Concentration | 2X |
| À utiliser avec (application) | RT-PCR |
Invitrogen™ PureLink™ PCR Purification Binding Buffer B2
PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
| Compatibilité à haut débit | High-throughput Compatible |
|---|---|
| Durée de fonctionnement | 5 min |
| Format | Kit |
| Technologie d’isolement | ChargeSwitch™ Chemistry |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Type de produit | PCR Clean-Up Kit |
| Gamme de produits | ChargeSwitch |
| Type d’échantillon | PCR Products |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| Contenu et stockage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
| Méthode de détection | SYBR |
|---|---|
| À utiliser avec (équipement) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Vitesse de réaction | Fast |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | UP (Ultra Pure) |
| Type d’échantillon | Cells |
| Concentration | 20X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | RT-PCR |
| Contenu et stockage | Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature. |
| Polymérase | AmpliTaq Fast DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Gamme de produits | Cells-to-CT, SYBR |
| Transcriptase inverse | M-MLV |
| Temps de purification | 5 min. |
| Marqueur ou colorant | SYBR Green |
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.