missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. Direct PCR Reagents and Kits
  7. 4458235

Invitrogen™ Single Cell Lysis Kit
GREENER_CHOICE

Catalog No. 4458235
Encompass
Change view
Click to view available options
Quantity:
100 preps
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
4458235 100 preps
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 4458235 Supplier Invitrogen™ Supplier No. 4458235
Only null left

Description

Includes

100μL Single Cell Dnase I, 1mL Single Cell Lysis Solution, 100μL Single Cell Stop Solution

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

  • Optimized sample prep—Enables RNA preparation for single cells
  • Efficient design—No sample loss ensures maximal sensitivity
  • Versatile application—Compatible with common enzymatic reactions

Optimized RNA Sample Prep for 1–10 Cells
The Invitrogen Single Cell Lysis Kit enables RNA preparation from 1 to 10 cells. The Single Cell Lysis kit generates a concentrated cell lysate through the optimized combination of lysis buffer, stop solution, and DNase. The resulting lysate is compatible with common enzymatic reactions such as reverse transcription. Sample preparation is fast and can be completed in only 7 minutes with two pipetting steps.

No Sample Loss Ensures Maximal Sensitivity
The Invitrogen Single Cell Lysis Kit bypasses the need for traditional RNA purification. There are no sample transfers, columns or washes which can lead to loss and/or dilution of the precious sample. The entire lysate can be used in downstream reactions ensuring maximal sensitivity and accuracy in your experiments.

Compatible with Common Enzymatic Reactions
The reagents in the Invitrogen Single Cell Lysis Kit are identical to the lysis reagents in the complete Single Cell-to-CT™ Kit for Real-time RT-PCR analysis from 1 to 10 cells. They have been validated with multiple reverse transcription kits including SuperScript™ VILO™ cDNA Synthesis Kit, High Capacity RNA-to-cDNA kit and TaqMan™ MicroRNA Reverse Transcription Kit, for analysis of mRNA as well as miRNA targets. As a standalone module, the Single Cell Lysis Kit allows you to create new workflows to expand the possibilities of single cell analysis. 100 preps.

Not high-throughput compatible (Manual)

Specifications

Concentration 2X
Content And Storage Kit contains:
  • 100 μL Single Cell Dnase I: -20°C;
  • 1 mL Single Cell Lysis Solution: 4°C;
  • 100 μL Single Cell Stop Solution: -20°C
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line Ambion, Cells-to-CT
Purification Time 7 min.
Quantity 100 preps
Shipping Condition Dry Ice
Target Lysate, ready for real-time PCR
For Use With (Application) Real Time PCR (qPCR), RT-PCR
Final Product Type Lysate
No. of Reactions 100 preps
Sample Type Bacteria, Blood, Cells, Tissue, Viral Samples
Starting Material 1-10 cells
Show More Show Less

Frequently Asked Questions (FAQs)

What is the minimum amount of cells required to perform RNA isolation using the Single Cell Lysis Kit?

The Single Cell Lysis Kit enables RNA preparation from 1-10 cells. It generates a concentrated cell lysate through the optimized combination of lysis buffer, stop solution, and DNase. The resulting lysate is compatible with common enzymatic reactions such as reverse transcription. Sample preparation is fast and can typically be completed in around 7 minutes with 2 pipetting steps.
Can laser micro-dissected FFPE single cells be used with a Single Cell-to-CT Kit?

While we have not tried this in-house, there is reason to believe this should work. However, optimization of the protocol around the RT step may be needed, as FFPE samples have a lot of RNA crosslinking, which could affect the efficiency of the RT enzyme. Heating the sample for 10 min at 80 degrees C before adding the RT in order to get good cDNA conversion should improve reverse transcription.

For Research Use Only. Not for use in diagnostic procedures.