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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Cytiva HyrTrp Cell Dissociation Reagent

Recombinant and animal-derived component-free alternative to traditional porcine trypsin

Content And Storage -20 °C
For Use With Attachment-dependent cell types MRC-5, Vero, CHO K-1 cell lines grown with or without serum
Product Type Cell Culture Dissociation Reagent
Shelf Life 12 months from manufacturing date when stored at -20°C
2

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Content And Storage -20°C
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Calcium,No Magnesium,No Sodium Bicarbonate
pH 7 to 7.6
Sterility Sterile
3

Invitrogen™ Accutase - Enzyme Cell Detachment Medium

Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.

4

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

5

Gibco™ Trypsin/EDTA Solution (TE)  DFS Item
GREENER_CHOICE

With EDTA and phenol red

6

MilliporeSigma™ ESGRO Complete™ Accutase™ Cell Detachment Solution

a cell detachment solution of proteolytic and collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

9

Gibco™ Dispase II, powder  DFS Item

Amino-endo peptidase that hydrolyzes N-terminal peptide bonds of non-polar amino acid residues

10

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red  DFS Item
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
11

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

12

Gibco™ Cell Dissociation Buffer, enzyme-free, PBS  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

15

Gibco™ CTS™ Versene™ Solution  DFS Item

CTS Versene Solution is a cGMP-manufactured EDTA solution for use as a gentle non-enzymatic cell dissociation reagent.