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Promega E. coli S30 Extract System for Circular DNA
E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors.
Supplier: Promega L1020
Includes:
S30 Extract, Circular, 3 x 150μL; Luciferase Dilution Reagent, 1mL; pBESTluc™ DNA, Circular (Control), 10μg; Luciferase Assay Reagent, 250μL; S30 Premix without Amino Acids, 750μL; Amino acid mixtures, 3 x 175μL
Description
- A Powerful Tool for Identifying and Characterizing Polypeptides
- Reduced chance of expressed proteins degrading
- Includes all components needed for coupled transcription/translation
- Produces very low levels of endogenous proteins, thus low backgrounds
- U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289 and other patents and patents pending.
- The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Greener Choice Claims
- Environmental benefits include:
- Recyclable
Specifications
<-65°C | |
3 x 150μL S30 circular extract, 1mL Luciferase Dilution Reagent, 10μg pBESTluc™ DNA, Linear (control), 250μL Luciferase Assay Reagent, 750μL S30 premix without Amino | |
450 μL |
Regulation of prokaryotic gene expression, identification of cloned DNA gene products or mapping of polypeptides to defined DNA fragments, rapid screening for translation from eukaryotic or prokaryotic DNAs cloned in vectors designed for high expression in E. coli, screening of compounds for inhibition of bacterial translation | |
30 |