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Promega™ HaloLink™ Protein Array Systems

Provides a convenient way to create protein array

Manufacturer: promega™  G4491

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TNT T7 Quick Coupled Transcription/Translation System or TNT SP6 High-Yield Wheat Germ Protein Expression System to use HaloLink Array Six Slide System

Catalog No. PRG4491


Description & Specifications


Quantity 30μg
Type Standard Protein
Format Liquid
For Use With (Equipment) HaloLink™ Protein Array System
For Use With (Application) Protein : protein interactions, protein: drug interactions, protein: nucleic acid interactions, antibody immobilization
Includes Two slide system contains HaloLink slides, HaloLink array gaskets for creating 50-well arrays, a HaloTag standard Protein, anti-HaloTag Antibody and cell-free expression system (TNT™ T7 Quick coupled transcription/translation system or T
pH 7.3
Storage Requirements -20°C

Using the HaloLink Protein Array Systems, HaloTag™ fusion proteins are expressed in cell-free expression system and then covalently captured on hydrogel-coated glass slides derivatized with HaloTag ligands. Fusion proteins are captured directly from expression reaction mixture without prior purification. Using this approach, multiple fusion proteins can be rapidly synthesized and immobilized in parallel on slide surface, and entire experiment including protein expression, custom array formation and protein interaction analysis can be completed in less than eight hours.

  • Cell-free expression systems allow quick, single-tube, coupled transcription/translation for production of proteins of interest to be used in protein array experiment
  • HaloTag fusion proteins are covalently bound to HaloLink Slide, providing irreversible binding of captured protein
  • Protein of interest is immobilized directly from cell-free expression reaction, no protein purification step required
  • Slides have hydrogel coating to prevent nonspecific binding while preserving functionality of specifically captured proteins
  • 50-well configuration for multiple interactions to be studied in parallel without robotic arrayer
HaloTag Standard protein
  • Mutated hydrolase
  • Forms covalent bond with HaloTag ligands
HaloTag Fusion protein
  • Consists of recombinant Protein G from Streptococci without albumin-binding domain and HaloTag protein
  • Molecular weight: 58kDa
  • Can be covalently coupled to different chloroalkane surfaces, reactive and fluorescent HaloTag ligands

Protein:protein, protein:drug or protein:nucleic acid interactions