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Thermo Scientific™ Pierce™ Recombinant Protein G and Conjugates

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Manufacturer:  Thermo Scientific™ 77675

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Catalog No. PI77675



Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.


  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA


Salt-free powder