Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Supplier: Thermo Scientific™ SO181
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases, such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.
Features
• 3'-terminal PTO modifications protect from 3'→5' exonuclease degradation and ensure efficient priming of DNA synthesis
• Product delivered in a ready-to-use format
Applications
• Strand displacement amplification of genomic DNA, plasmids, and phage DNA
• DNA labeling by random priming
| Primer | |
| Dry Ice | |
| Random | |
| DNA Labeling, Strand Displacement Amplification |
| 20X Exo-Resistant Random Primer (100 μL) Store at –20°C. |
|
| 500 μM | |
| 100 μL | |
| Liquid |
For Research Use Only. Not for use in diagnostic procedures.
