Thermo Scientific™ Exo-Resistant Random Primer
Perform highly efficient random priming of DNA synthesis reactions with this mixture of single-stranded random oligonucleotides.
Manufacturer: Thermo Scientific™ SO181
Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases , such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.
- 3'-terminal PTO modifications protect from 3'→5' exonuclease degradation and ensure efficient priming of DNA synthesis
|Strand displacement amplification of genomic DNA (2), plasmids and phage DNA (3); DNA labeling by random priming (4–6).|
|Exo-Resistant Random Primer|