Real Time PCR Master Mixes
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Thermo Scientific™ Maxima Probe qPCR Master Mixes
Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.
Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial
Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.
| Polymérase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon |
| Vitesse de réaction | Standard |
| Format | Tube |
| Type de produit | qPCR Master Mix |
| Gamme de produits | Maxima |
| Type d’échantillon | DNA, dsDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
| Thermostabilité | 72 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 3-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | ROX (Pre-mixed) |
| Type d’échantillon | DNA |
| InhibitorToleranceSimple | Very High |
| Méthode PCR | qPCR |
| À utiliser avec (application) | Microbial Detection |
| Contenu et stockage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
| Gamme de produits | TaqPath |
| Sensibilité | Down to 1 copy |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | cDNA or gDNA only |
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and 1-Step RT-dPCR Master Mix
A key component of the QuantStudio Absolute Q Digital PCR System which is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated sample separation.
Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
| Polymérase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast |
| Durée d’activation | 5 min. |
| Hot Start | Built-in Hot Start |
| Type de produit final | Mastermix |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 2X |
| Compatibilité | TaqMan™ |
| Matériau de départ | DNA/RNA |
| À utiliser avec (application) | Animal Health |
| Marqueur ou colorant | ROX |
| Forme | Liquid |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
| Thermostabilité | 8 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 5-plex |
| GC-Rich PCR Performance | Medium |
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | ROX (Pre-mixed) |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
| Forme | Liquid |
| Contenu et stockage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Polymérase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Hot Start | Antibody-based |
| Gamme de produits | TaqPath |
| Comprend | dUTP |
| Durée de conservation | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Spécificité cible | RNA only, Both RNA and DNA |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
| Thermostabilité | 8 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 6-plex |
| GC-Rich PCR Performance | Medium |
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | None |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
| Forme | Liquid |
| Contenu et stockage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Polymérase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Hot Start | Antibody-based |
| Gamme de produits | TaqPath |
| Comprend | dUTP |
| Durée de conservation | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Spécificité cible | RNA only, Both RNA and DNA |
Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)
Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.
Fisherbrand™ SYBR™ Green qPCR Master Mix
Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| Vitesse de réaction | Fast or Standard |
| Type de produit | Master Mix |
| Méthode PCR | qPCR,2-step RT-qPCR,RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
| Marqueur ou colorant | SYBR Green,FAM |
| Forme | Frozen |
| Contenu et stockage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox
TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
| Thermostabilité | 72 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 4-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™ 12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | None |
| Type d’échantillon | DNA |
| InhibitorToleranceSimple | Very High |
| Méthode PCR | qPCR |
| À utiliser avec (application) | Microbial Detection |
| Gamme de produits | TaqPath |
| Sensibilité | Down to 1 copy |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | cDNA or gDNA only |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
| Thermostabilité | 72 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Single-plex |
| Méthode de détection | SYBR |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Vitesse de réaction | Fast or Standard |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Type d’échantillon | DNA |
| InhibitorToleranceSimple | Medium |
| Méthode PCR | qPCR |
| Concentration | 2X |
| Conditions d’expédition | Wet Ice |
| À utiliser avec (application) | Gene Expression |
| Polymérase | Dual-Lock Taq DNA Polymerase |
| Format | Tube |
| Gamme de produits | PowerUP, SYBR |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | cDNA or gDNA only |
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix
TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
| Thermostabilité | Not Applicable |
|---|---|
| Capacité multiplex | Up to 4-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx |
| Vitesse de réaction | Fast or Standard |
| Type de produit | qPCR Master Mix |
| Colorant de référence passive | Mustang Purple |
| Type d’échantillon | DNA, RNA |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression,Microbial Detection |
| Polymérase | Fast DNA Polymerase |
| Format | Tube |
| Gamme de produits | TaqPath |
| Sensibilité | Down to 10 copies |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | RNA only, Both RNA and DNA |
Applied Biosystems™ TaqPath™ ProAmp™ Master Mix
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.
| Thermostabilité | 72 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 3-plex, Up to 4-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System |
| Vitesse de réaction | Fast or Standard |
| Type de produit | qPCR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| InhibitorToleranceSimple | Very High |
| Méthode PCR | qPCR |
| Conditions d’expédition | Wet Ice |
| À utiliser avec (application) | Genotyping |
| Contenu et stockage | Store at 2°C to 8°C in the dark. |
| Polymérase | Taq DNA Polymerase |
| Gamme de produits | TaqPath |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | cDNA or gDNA only |