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  7. Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Catalog No. FERK0261
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200 Reactions
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Catalog No. No. of Reactions
FERK0261 200 Reactions
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Catalog No. FERK0261 Supplier Thermo Scientific™ Supplier No. K0261
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Description

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA polymerase and dNTPs in an optimized PCR buffer. ROX Solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima Hot Start Taq DNA polymerase in combination with the optimized buffer ensures PCR specificity and sensitivity.dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mix in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity—detects low copy number targets
  • ROX Solution provided in a separate tube
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Universal—can be used on most real-time PCR instruments
  • Reproducibility and convenience—ready-to-use 2X master mix

Recommended for:

Real-time PCR and RT-qPCR using sequence specific probes on most real-time PCR machines.

Specifications

Concentration 2X
Content And Storage Contains:
• 2 x 1.25 mL Maxima Probe qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer; sufficient for 200 x 25 μL reactions.
• 50 μL ROX solution, 50 μM
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Line Maxima
Product Type qPCR Master Mix
Quantity 200 reactions
Sufficient For 200 Reactions of 25 μL
For Use With (Application) Gene Expression
No. of Reactions 200 Reactions
Sample Type DNA, dsDNA
Volume 2 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.