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Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
$201.00 - $7350.00
Specifications
Detection Method | SYBR |
---|---|
Format | Tube |
GC-Rich PCR Performance | High |
PCR Method | qPCR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.
Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
4344463
|
Applied Biosystems™
4344463 |
1 x 1 mL |
Each for $201.00
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4309155
|
Applied Biosystems™
4309155 |
1 x 5 mL |
Each for $752.00
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4364344
|
Applied Biosystems™
4364344 |
2 x 5 mL |
Each for $1,366.00
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4364346
|
Applied Biosystems™
4364346 |
5 x 5 mL |
Each for $2,950.00
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4312704
|
Applied Biosystems™
4312704 |
10 x 5 mL |
Each for $7,350.00
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4334973
|
Applied Biosystems™
4334973 |
1 x 50 mL |
Each for $7,230.00
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Description
Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
- AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
- dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
- Proprietary buffer enhancements ensure performance and reliability
- Premixed components stored at 2° to 8°C significantly reduce assay setup time
Maximum Flexibility and Convenience:
- Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
- SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
- The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
- Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
- SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
- Store at -20°C
Order Info
Shipping Condition: Wet Ice
Specifications
SYBR | |
High | |
AmpliTaq Gold DNA Polymerase | |
SYBR Green | |
SYBR™ | |
Gene Expression |
Tube | |
qPCR | |
Standard | |
ROX (Pre-mixed) | |
Real Time PCR SYBR Master Mix | |
2X |
For Research Use Only. Not for use in diagnostic procedures.