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Applied Biosystems™ SYBR™ Green Universal Master Mix 
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
$227.00 - $8290.00
Specifications
| Concentration | 2X |
|---|---|
| Detection Method | SYBR |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|
4344463
|
Applied Biosystems™
4344463 |
1 x 1 mL |
Each for $227.00
|
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|
4309155
|
Applied Biosystems™
4309155 |
1 x 5 mL |
Each for $848.00
|
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|
4364344
|
Applied Biosystems™
4364344 |
2 x 5 mL |
Each for $1,542.00
|
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|
4364346
|
Applied Biosystems™
4364346 |
5 x 5 mL |
Each for $3,330.00
|
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|
4312704
|
Applied Biosystems™
4312704 |
10 x 5 mL |
Each for $8,290.00
|
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|
4334973
|
Applied Biosystems™
4334973 |
1 x 50 mL |
Each for $8,160.00
|
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Description
Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
- AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
- dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
- Proprietary buffer enhancements ensure performance and reliability
- Premixed components stored at 2° to 8°C significantly reduce assay setup time
Maximum Flexibility and Convenience:
- Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
- SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
- The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
- Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
- SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
- Store at -20°C
Order Info
Shipping Condition: Wet Ice
Specifications
| 2X | |
| Tube | |
| qPCR | |
| Standard | |
| ROX (Pre-mixed) | |
| Real Time PCR SYBR Master Mix |
| SYBR | |
| High | |
| AmpliTaq Gold DNA Polymerase | |
| SYBR Green | |
| SYBR | |
| Gene Expression |
For Research Use Only. Not for use in diagnostic procedures.