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Applied Biosystems™ SYBR™ Green PCR Master Mix DFS Item

Everything needed for SYBR Green dye-based PCR amplification and detection in a convenient, single-tube format

$141.75 - $5,244.75

Specifications

For Use With (Application) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
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Includes:  2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

4309155

DFS Item
 
life technologies
4309155
1 x 5mL Each for $535.50

4312704

DFS Item
 
life technologies
4312704
10 x 5mL Each for $5,244.75

4334973

DFS Item
 
life technologies
4334973
1 x 50mL Each for $5,055.75

4344463

DFS Item
 
life technologies
4344463
1 x 1mL Each for $141.75

4364344

DFS Item
 
life technologies
4364344
2 x 5mL Each for $972.30

4364346

DFS Item
 
life technologies
4364346
5 x 5mL Each for $2,100.00
Description & Specifications

Specifications

For Use With (Application) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System

SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

  • This mix combines SYBR Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
  • Premixed components significantly reduce assay setup time
  • SYBR Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required. SYBR Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR-related fluorescence fluctuations which minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation.

Gene Expression Analysis & Genotyping, PCR & Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling

Shipping Condition: Wet Ice

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