missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Applied Biosystems™ SYBR™ Green PCR Master Mix DFS Item

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format.

$147.00 - $5,400.00

Specifications

Detection Method Detection Method: SYBR; PCR Method: qPCR
Speed Standard
Sample Type DNA (Genomic), cDNA
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
For Use With (Application) Real time PCR (qPCR), real time PCR-based gene expression profiling
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
4309155 Applied Biosystems™
LS4309155
1 x 5mL Each for $552.00
4312704 Applied Biosystems™
LS4312704
10 x 5mL Each for $5,400.00
4334973 Applied Biosystems™
LS4334973
1 x 50mL Each for $5,210.00
4344463 Applied Biosystems™
LS4344463
1 x 1mL Each for $147.00
4364344 Applied Biosystems™
LS4364344
2 x 5mL Each for $1,002.00
4364346 Applied Biosystems™
LS4364346
5 x 5mL Each for $2,165.00
Specifications

Description

Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • Premixed components stored at 2° to 8°C significantly reduce assay setup time
  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience:
  • Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

    Order Info

    Shipping Condition: Wet Ice

Description & Specifications

Specifications

Detection Method: SYBR; PCR Method: qPCR
DNA (Genomic), cDNA
Real time PCR (qPCR), real time PCR-based gene expression profiling
Standard
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Store at 2° to 8°C.