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Invitrogen™ Staphylococcus aureus (Wood strain without protein A) BioParticles™, fluorescein conjugate

Catalog No. S2851
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S2851 10 mg
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Catalog No. S2851 Supplier Invitrogen™ Supplier No. S2851
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Description

Fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities

  • Fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities
  • Fluorescent BioParticles products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry
  • Fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor™, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformLy intense over the pH range from 3 to 10
  • Label (Ex/Em): Fluorescein (approx. 494/518nm)
  • Particle: S. aureus (Wood strain, without protein A)
  • Provided as lyophilized powders
  • Approximately 3 x 108 S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid
  • Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
  • Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
  • Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutant s
  • Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Structure, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Receptors

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope
Product Type Bioparticle Conjugate
Dye Type FITC (Fluorescein)
Form Lyophilized Powder
pH 3 to 10
Product Line BioParticles
Quantity 10 mg
Shipping Condition Room Temperature
Species S. aureus
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Frequently Asked Questions (FAQs)

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.
What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

For Research Use Only. Not for use in diagnostic procedures.