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Gibco™ StemPro™ Chondrogenesis Differentiation Kit

Catalog No. A1007101
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Catalog No. A1007101 Supplier Gibco™ Supplier No. A1007101
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Description

StemPro™ Chondrogenesis Differentiation Kit

The StemPro™ Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the chondrogenesis pathway and generate chondrocytes. Using StemPro™ Chondrogenesis Differentiation Kit in combination with StemPro™ MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion, and differentiation into collagen matrix-producing chondrocytes.

Specifications

Cell Type Stem Cells (Mesenchymal)
Content And Storage This kit is shipped with a 100 ml basal medium and 10 ml growth supplement adequate to formulate 100 ml of complete medium. Components require separate shipping and storage requirements. The growth supplement is not sold individually.
StemPro™ Osteocyte⁄Chondrocyte Differentiation Basal Medium 2 to 8°C (protect from light)
StemPro™ Chondrogenesis Supplement -5 to -20°C (in the dark)
Culture Environment CO2
For Use With (Application) Stem Cell Differentiation
Product Type Chondrogenesis Differentiation Kit
Endotoxin Level ≤50 EU/mL, ≤ 50 EU/ml
Format Liquid
Glucose Low Glucose
Glutamine GlutaMAX™-I
HEPES Buffer No HEPES
Phenol Red Indicator No Phenol Red
Product Line Gibco, StemPro
Purity or Quality Grade Research Grade
Quantity 1 Kit
Research Category Differentiation
Serum Level Serum-Free
Sodium Bicarbonate Buffer Sodium Bicarbonate
Sodium Pyruvate Additive Sodium Pyruvate
Species Human
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Frequently Asked Questions (FAQs)

How many times can I freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A1006401) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

We do not recommend that you freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A10064-01) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101). Addition of supplement to the StemPro Osteocyte/Chondrocyte Differentiation Basal Medium (Cat. No. A1006901) is stable at 2-8 degrees C for up to 1 month, protected from light.

What mesenchymal stem cells (MSC) sources can I use with the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

The StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101) was designed and tested using human adipose-derived stem cells (ADSC). Based on protocols in literature, it should also work with mesenchymal stem cells (MSC) from other sources, including umbilical cord and cord blood.

The StemPro Chondrogenesis Differentiation Kit manual refers to MSC Attachment Medium under Chondrogenesis Differentiation, Step 7. Is MSC Attachment Medium the same as MSC growth medium?

Yes, MSC Attachment Medium is the same as MSC growth medium.
What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.
How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).
How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).
What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

For Research Use Only. Not for use in diagnostic procedures.