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Invitrogen™ JC-1 Dye (Mitochondrial Membrane Potential Probe)

Catalog No. T3168
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T3168 5 mg
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Catalog No. T3168 Supplier Invitrogen™ Supplier No. T3168
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Description

Sensitive marker for mitochondrial membrane potential

JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. The dye exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that exhibit a broad excitation spectrum and an emission maximum at ∽590nm.

Another dye with similar characteristics is JC-9 (D-22421).

Apoptosis, Cell Analysis, Cell Structure, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function, Mitochondria, Mitochondria Function, Mitochondrial Function

Order Info

Shipping Condition: Room Temperature

Specifications

Color Green
Content And Storage Store at room temperature and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Dye
Quantity 5 mg
Shipping Condition Room Temperature
Sub Cellular Localization Mitochondria

Frequently Asked Questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.
What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

For Research Use Only. Not for use in diagnostic procedures.