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Invitrogenā¢ CellTraceā¢ Violet Cell Proliferation Kit, for flow cytometry 
CellTraceā¢ Violet Cell Proliferation Kit, for flow cytometry
Supplier: Invitrogenā¢ C34571
Description
CellTraceā¢ Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.View a selection guide for all CellTraceā¢ Cell Proliferation Kits for flow cytometry.
Superior fluorescent staining
Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTraceā¢ Violet dye enables the visualization of ten or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.
Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTraceā¢ Violet stain easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye provides a consistent signal, even after several days in a cell culture environment. CellTraceā¢ Violet dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells.
Easy multiplexing with other fluorophores
The violet excitation and narrow emission of CellTraceā¢ Violet dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluorā¢ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry) (see Fluorescence Spectra for CellTraceā¢ Violet stain below).
Simple, robust staining protocol
The CellTraceā¢ Violet Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1Ī¼L of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.
Specifications
| Contains 1 vial of CellTraceā¢ Violet reagent and 1 vial of DMSO (100 Ī¼L). |
|
| Proliferation Assay | |
| Cell Proliferation Kit | |
| 405/450 | |
| Tube(s) | |
| 20 Assays | |
| Room Temperature |
| Fluorescence | |
| Flow Cytometer | |
| Contains 1 vial of CellTraceā¢ Violet reagent and 1 vial of DMSO (100Ī¼L) | |
| Solid | |
| CellTrace | |
| CFSE & Related Compounds | |
| DMSO (Dimethylsulfoxide) |
For Research Use Only. Not for use in diagnostic procedures.